电针经Urocortin1/CRF-R1通路缓解功能性消化不良大鼠十二指肠微炎症*

作者：陈皖倩，康朝霞，崔冬梅

单位：贵州中医药大学针灸推拿学院，贵州 贵阳 550025

引用：引用：陈皖倩，康朝霞，崔冬梅.电针经Urocortin1/CRF-R1通路缓解功能性消化不良大鼠十二指肠微炎症[J].中医药导报,2026,32(4):30-35,47.

DOI：10.13862/j.cn43-1446/r.2026.04.006

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摘要：

目的：探讨电针通过尿皮质素1（Urocortin 1，Ucn1）/促肾上腺皮质激素释放因子受体1（CRF-R1）通路对功能性消化不良（FD）模型大鼠十二指肠组织微炎症的影响。方法：从80只SD大鼠中随机抽取10只为空白组，其余70只采用多因素干预法制备FD模型。造模成功后，分为模型组、电针组、Ucn1组、Ucn1+电针组，每组10只。电针组于“后三里”穴行电针干预，Ucn1组给予尾静脉注射Ucn1（10 μg/kg），Ucn1+电针组则同时接受两种干预，均持续14 d。检测指标包括一般行为评分、小肠推进率、血清白细胞介素-10（IL-10）含量、十二指肠类胰蛋白酶表达，以及下丘脑和十二指肠中Ucn1/CRF-R1的表达与共定位。结果：与模型组比较，电针组大鼠干预后的一般情况评分明显增高（P＜0.01），类胰蛋白酶表达量明显降低（P＜0.01），小肠推进率、血清IL-10含量明显升高（P＜0.01）；Ucn1组大鼠一般情况评分无明显差异（P＞0.05），血清IL-10含量明显降低，小肠推进率、类胰蛋白酶表达量均明显增高（P＜0.01）；Ucn1+电针组大鼠一般情况评分、类胰蛋白酶表达量无显著性差异（P＞0.05），小肠推进率明显增高（P＜0.01），血清IL-10含量明显降低（P＜0.01）。与电针组比较，Ucn1组大鼠一般情况评分、血清IL-10含量均明显降低（P＜0.01），小肠推进率、类胰蛋白酶表达量均明显增高（P＜0.01）；Ucn1+电针组一般情况评分无明显差异（P＞0.05），血清IL-10含量明显降低（P＜0.01），小肠推进率、类胰蛋白酶表达量均明显增高（P＜0.01）。与Ucn1组比较，Ucn1+电针组一般情况评分、血清IL-10含量明显增高（P＜0.01），小肠推进率、类胰蛋白酶表达量均明显降低（P＜0.01）。结论：电针可能通过调控十二指肠Ucn1/CRF-R1通路缓解FD大鼠十二指肠组织微炎症。

关键词：功能性消化不良；电针；“后三里”穴；尿皮质素1；促肾上腺皮质激素释放因子受体1；十二指肠；微炎症；大鼠

Abstract：

Objective: To investigate the effect of electroacupuncture on micro-inflammation in duodenal tissues of functional dyspepsia (FD) model rats through urocortin1 (Ucn1)/corticotropin-releasing factor receptors 1 (CRF-R1) pathway. Methods: A total of 10 SD rats were randomly selected from 80 SD rats as the blank control group , and the remaining 70 were used to establish a FD model using a multi-factor intervention method. After successful model establishment, the rats were further divided into a model group, aelectroacupuncture (EA) group, a Ucn1 group, and a Ucn1+EA group, 10 rats in each group. The EA group received electroacupuncture stimulation at the "Housanli" (后三里) point. The Ucn1 group received tail vein injection of Ucn1 (10 μg/kg). The Ucn1+EA group received both interventions simultaneously. All interventions lasted for 14 days. Measurements included general behavioral scores, small intestinal propulsion rate, serum interleukin-10 (IL-10) level, duodenal tryptase expression, as well as the expression and co-localization of Ucn1/CRF-R1 in the hypothalamus and duodenum. Results: Compared with the model group, the EA group exhibited significantly increased general condition scores (P<0.01), and significantly reduced tryptase expression (P<0.01), as well as significantly elevated small intestinal propulsion rate and serum IL-10 level. The Ucn1 group showed no significant difference in general condition scores (P>0.05), significantly reduced serum IL-10 levels, and significantly increased small intestinal propulsion rate and tryptase expression (P<0.01). The Ucn1+EA group demonstrated no significant differences in general condition score and tryptase expression (P>0.05), but significantly increased small intestinal propulsion rate (P<0.01) and significantly decreased serum IL-10 level (P<0.01). Compared with the EA group, the Ucn1 group showed significantly lower general condition scores and serum IL-10 level (P<0.01), along with significantly higher small intestinal propulsion rate and tryptase expression (P<0.01). The Ucn1+EA group showed no significant difference in general condition score (P>0.05), but significantly lower serum IL-10 level (P<0.01) and significantly higher small intestinal propulsion rate and tryptase expression (P<0.01). Compared with the Ucn1 group, the Ucn1 + EA group displayed significantly higher general condition scores and serum IL-10 level (P<0.01), and significantly lower small intestinal propulsion rate and tryptase expression (P<0.01). Conclusion: Electroacupuncture may ameliorate micro-inflammation in duodenal tissues of FD rats by modulating the duodenal Urocortin1/CRF-R1 pathway.

Key words：functional dyspepsia; electroacupuncture; "Housanli"; urocortin 1; corticotropin-releasing factor receptor 1; duodenum; micro-inflammation; rat

发布时间：2026-04-26

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