五谷麒麟膏调控糖酵解促进肛瘘术后大鼠创面愈合*

作者：郭 萌1，兰 雯1，石 鹏2

单位：1.陕西中医药大学，陕西 咸阳 712046； 2.陕西中医药大学附属医院，陕西 咸阳 712000

引用：引用：郭萌，兰雯，石鹏.五谷麒麟膏调控糖酵解促进肛瘘术后大鼠创面愈合[J].中医药导报,2026,32(3):32-37.

DOI：10.13862/j.cn43-1446/r.2026.03.006

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摘要：目的：探讨五谷麒麟膏对肛瘘术后大鼠创面愈合的影响，并从调控丙酮酸激酶M2（PKM2）及白细胞介素-10（IL-10）表达的角度阐明其潜在分子机制。方法：建立雄性SD大鼠肛瘘术后创面模型。将60只大鼠随机分为空白组、模型组、康复新液组、五谷麒麟膏低剂量组、五谷麒麟膏中剂量组及五谷麒麟膏高剂量组，每组10只。各组分别给予相应处理，连续换药14 d。通过计算创面愈合比较各组创面愈合情况，HE染色观察给药后14 d各组创面组织病理形态，实时荧光定量PCR（Real-time PCR）检测各组创面组织中IL-10表达水平，蛋白质印迹法（Western bloting）检测各组创面组织中PKM2蛋白表达情况。结果：五谷麒麟膏各剂量组均能显著加速大鼠创面愈合，创面愈合率均高于空白组与模型组，炎症细胞浸润程度低于空白组及模型组，肉芽组织成熟度与上皮再生程度优于空白组及模型组。分子机制研究显示，在创面愈合早期，五谷麒麟膏各剂量组的IL-10 mRNA表达量低于模型组（P＜0.05），五谷麒麟膏各剂量组PKM2蛋白表达高于模型组（P＜0.01）；在创面愈合中期，五谷麒麟膏高、中剂量组的IL-10 mRNA表达量高于模型组（P＜0.05），同时，五谷麒麟膏各组的PKM2蛋白表达水平均低于模型组（P＜0.01）；在创面愈合后期，五谷麒麟膏高、中剂量组及康复新液组的IL-10 mRNA表达量高于模型组（P＜0.01），五谷麒麟膏各剂量组、康复新液组的PKM2蛋白表达水平均低于模型组（P＜0.01）。结论：五谷麒麟膏能有效促进肛瘘术后大鼠的创面愈合，其作用机制可能与动态调控创面微环境的代谢状态有关。

关键词：肛瘘；创面愈合；五谷麒麟膏；康复新液；糖酵解；丙酮酸激酶M2；白细胞介素-10；大鼠

Abstract：

Objective: To investigate the effects of Wugu Qilin Ointment on wound healing in rats after anal fistula surgery and to elucidate its potential molecular mechanisms through the regulation of the expression of pyruvate kinase M2 (PKM2) and interleukin-10 (IL-10). Methods: A postoperative anal fistula wound model was established in male SD rats. Sixty rats were randomly divided into a blank group, a model group, a Kangfuxin Liquid (康复新液) group, a low-dose Wugu Qilin Ointment group, a medium-dose Wugu Qilin Ointment group, and a high-dose Wugu Qilin Ointment group, with 10 rats in each group. Each group received the corresponding treatment, and dressings were changed continuously for 14 days. Wound healing in each group was compared by calculating the wound healing rate. Histopathological morphology of wound tissues on day 14 post-treatment was observed using HE staining. IL-10 expression levels in wound tissues were detected by real-time quantitative PCR (Real-time PCR), and PKM2 protein expression in wound tissues was assessed by Western blotting. Results: All dose groups of Wugu Qilin Ointment significantly accelerated wound healing in rats. The wound healing rates were higher than those in the blank group and the model group. The degree of inflammatory cell infiltration was lower than that in the blank group and model group, and granulation tissue maturity and epithelial regeneration were superior to those in the blank group and model group. Molecular mechanism studies showed that in the early stage of wound healing, IL-10 mRNA expression in all Wugu Qilin Ointment groups was lower than that in the model group (P<0.05), while PKM2 protein expression was higher than that in the model group (P<0.01). In the intermediate stage, IL-10 mRNA expression in the high-dose Wugu Qilin Ointment group and medium-dose Wugu Qilin Ointment group was higher than that in the model group (P<0.05), whereas PKM2 protein expression in all Wugu Qilin Ointment groups was lower than that in the model group (P<0.01). In the late stage, IL-10 mRNA expression in the high-dose Wugu Qilin Ointment group, medium-dose Wugu Qilin Ointment group and Kangfuxin Liquid group was higher than that in the model group (P<0.01), and PKM2 protein expression in all Wugu Qilin Ointment groups and Kangfuxin Liquid group was lower than that in the model group (P<0.01). Conclusion: Wugu Qilin Ointment can effectively promote wound healing in rats after anal fistula surgery, and its mechanism of action may be related to the dynamic regulation of the metabolic state of the wound microenvironment.

Key words：anal fistula; wound healing; Wugu Qilin Ointment; Kangfuxin Liquid; glycolysis; pyruvate kinase M2; interleukin-10; rats

发布时间：2026-03-28

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