正交试验法优化高良姜中槲皮素的提取工艺研究*

作者：林文新1，黄俊炜1，李儒亿1，阮清玲2，杨灿凤2，聂雨雯2，冯真英3

单位：1.广州市皮肤病防治所，广东 广州 510095； 2.岭南师范学院，广东 湛江 524048； 3.广东医科大学附属第二医院，广东 湛江 524001

引用：引用：林文新，黄俊炜，李儒亿，阮清玲，杨灿凤，聂雨雯，冯真英.正交试验法优化高良姜中槲皮素的提取工艺研究[J].中医药导报,2025,31(12):106-109,120.

DOI：10.13862/j.cn43-1446/r.2025.12.016

PDF： 下载PDF

摘要：目的：探讨超声辅助优化高良姜中槲皮素的提取工艺条件。方法：在单因素试验的基础上，采用高效液相色谱法定量分析槲皮素，通过正交试验筛选乙醇体积分数、超声时间及料液比对槲皮素含量的影响。结果：高良姜中槲皮素的最佳提取工艺条件为料液比1∶18（g/mL），超声时间（功率400 W，频率25 Hz）50 min，乙醇体积分数75%。在该工艺条件下，高良姜中槲皮素的提取量为88.15 μg/g。结论：优选的高良姜中槲皮素超声提取工艺合理、稳定、可行，可为高良姜中槲皮素的提取提供参考。

关键词：高良姜；槲皮素；单因素考察；正交试验；超声提取

Abstract：

Objective: To optimize the ultrasonic-assisted extraction conditions for quercetin from Gaoliangjiang (Alpinia officinarum Hance). Methods: Based on single-factor experiments, quercetin was quantitatively analyzed by high-performance liquid chromatography (HPLC). The effects of ethanol concentration, ultrasonic time, and solid-liquid ratio on quercetin content were screened using an orthogonal experiment. Results: The optimal extraction conditions were as follows: solid-liquid ratio of 1∶18 (g/mL), ultrasonic time of 50 min (power 400 W, frequency 25 Hz), and ethanol concentration of 75%. Under these conditions, the extraction yield of quercetin from Alpinia officinarum Hance was 88.15 μg/g. Conclusion: The optimized ultrasonic extraction process for quercetin from Alpinia officinarum Hance is reasonable, stable, and feasible, providing a reference for the extraction of quercetin from this herb.

Key words：Alpinia officinarum Hance; quercetin; single-factor investigation; orthogonal test; ultrasonic extraction

发布时间：2025-12-31

点击量：5