基于网络药理学及实验探讨益气活血消肿汤治疗乳腺癌术后淋巴水肿的作用机制*

作者：李嘉惠1，雷金敏1，沈新敏1，章 慧2

单位：1.湖南中医药大学，湖南 长沙 410208；2.湖南省肿瘤医院，湖南 长沙 410031

引用：引用：李嘉惠，雷金敏，沈新敏，章慧.基于网络药理学及实验探讨益气活血消肿汤治疗乳腺癌术后淋巴水肿的作用机制[J].中医药导报,2025,31(5):51-55,79.

DOI：10.13862/j.cn43-1446/r.2025.05.009

PDF： 下载PDF

摘要：目的：运用网络药理学技术预测益气活血消肿汤治疗乳腺癌术后淋巴水肿的作用机制及核心靶点，并通过实验验证益气活血消肿汤治疗乳腺癌术后淋巴水肿的机制。方法：使用中药系统药理学数据库与分析平台（TCMSP）、中医药百科全书（ETCM）数据库筛选益气活血消肿汤中药物活性成分及靶点，GeneCards、OMIM数据库获得疾病靶点，取交集后获得益气活血消肿汤治疗乳腺癌术后淋巴水肿的靶点，通过String数据库构建蛋白质相互作用网络，用Metascape数据库对作用靶点进行基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析，根据富集分析的结果进行实验验证。取7周龄SPF级C57BL/6J小鼠24只，随机分为模型组、中药组、西药组，每组8只。构建鼠尾淋巴水肿模型，造模成功后，开始予药物灌胃干预，模型组予0.9%氯化钠溶液，中药组予益气活血消肿汤，西药组予地奥司明片，连续灌胃21 d后，检测血清中白介素-1（IL-2）、γ干扰素（INF-γ）、IL-4、IL-10含量，对鼠尾组织进行HE染色及Masson染色分析。结果：网络药理学筛选出益气活血消肿汤的有效活性成分85个，药物和疾病的共同靶点64个，经拓扑分析，得到20个核心靶点，GO富集分析共得到1 454个条目，KEGG分析得到162个条目。动物实验结果显示，益气活血消肿汤可降低小鼠血清中IL-4、IL-10水平，提高IFN-γ、IL-2的水平，减少小鼠尾皮下组织厚度及皮下组织中胶原沉积，减轻皮下组织纤维化。结论：益气活血消肿汤可通过降低血清中IL-4、IL-10水平，提高IFN-γ、IL-2的水平，抑制Th2细胞分化，起到治疗乳腺癌术后淋巴水肿的作用。

关键词：乳腺癌术后；淋巴水肿；益气活血消肿汤；网络药理学；小鼠

Abstract：

Objective: To predict the mechanism and core target of Yiqi Huoxue Xiaozhong decoction in the treatment of postoperative breast cancer-related lymphedema with network pharmacology technology, and to verify the mechanism of Yiqi Huoxue Xiaozhong decoction in the treatment of postoperative breast cancer-related lymphedema through experiments. Methods: Traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP） and the encyclopedia of traditional Chinese medicine (ETCM) were used to screen the active ingredients and targets of drugs in Yiqi Huoxue Xiaozhong decoction. GeneCards and OMIM databases were used to obtain disease targets. After intersection, the targets of Yiqi Huoxue Xiaozhong decoction for the treatment of postoperative breast cancer-related lymphedema were obtained. Protein interaction maps were constructed through String database, and GO and KEGG enrichment analysis was performed on the target with Metascape database. Experimental verification was conducted according to the results of enrichment analysis. Totally 24 SPF grade C57BL/6J mice aged 7 weeks were randomly divided into model group, Chinese medicine group, and western medicine group, with 8 mice in each group. Mouse tail lymphedema model was constructed. After successful modeling, drug gavage intervention was initiated. The model group was given 0.9% physiological saline. The Chinese medicine group was given Yiqi Huoxue Xiaoxiao decoction, and the western medicine group was given Diosimin tablets. After continuous gavage for 21 days, the levels of IL-2, INF-γ, IL-4, and IL-10 in the serum were detected, and HE staining and Masson staining analysis were performed on the mouse tail tissue. Results: Network pharmacology screened 85 effective active ingredients of Yiqi Huoxue Xiaozhong decoction, 64 common targets of drugs and diseases, and obtained 20 core targets through topological analysis. GO enrichment analysis obtained a total of 1454 entries, and KEGG analysis obtained 162 entries. Animal experiment results showed that Yiqi Huoxue Xiaozhong decoction reduced the levels of IL-4 and IL10 in serum, increased the levels of IFN - γ and IL-2, reduced the thickness of subcutaneous tissue in mouse tail, decreased collagen deposition in subcutaneous tissue, and alleviated subcutaneous tissue fibrosis. Conclusion: Yiqi Huoxue Xiaozhong decoction can reduce the content of IL-4 and IL-10 in serum, increase the content of IFN-γ and IL-2, inhibit the differentiation of Th2 cells, and achieve the effect of treating postoperative breast cancer-related lymphedema.

Key words：postoperative breast cancer; lymphedema; Yiqi Huoxue Xiaozhong decoction; network pharmacology; mice

发布时间：2025-12-30

点击量：5