HPLC-DAD多波长切换法同时测定清肝化瘀方中9个成分含量*

作者：郭紫云1，杜丽娜1，张秋燕2，谢晓暄1，杨 燕1

单位：1.国家儿童医学中心/首都医科大学附属北京儿童医院，北京 100045； 2.中国中医科学院广安门医院，北京 100053

引用：引用：郭紫云，杜丽娜，张秋燕，谢晓暄，杨燕.HPLC-DAD多波长切换法同时测定清肝化瘀方中9个成分含量[J].中医药导报,2025,31(4):73-76,108.

DOI：10.13862/j.cn43-1446/r.2025.04.012

PDF： 下载PDF

摘要：

目的：采用高效液相色谱-二极管阵列检测器（HPLC-DAD）法建立清肝化瘀方多成分含量的测定方法。方法：采用Agela ASB C18色谱柱（5.0 μm，4.6 mm×250.0 mm），以乙腈（A）-0.4%磷酸水溶液（B），梯度洗脱0～10 min，5%A~15%A；10～20 min，15%A~20%A；20～45 min，20%A~30%A；45~60 min，30%A~70%A，柱温为25 ℃，流速为1.0 mL/min，进样量为10 μL，检测波长分别为210 nm（0~11 min，丹参素钠）、327 nm（11~24 min，绿原酸、新绿原酸、隐绿原酸）、292 nm（24~35 min，落新妇苷）、286 nm（35~42 min，丹酚酸B）、370 nm（42~60 min，槲皮素、山柰酚）及290 nm（50~52.5 min，柚皮素）。结果：丹参素钠、丹酚酸B、槲皮素、山柰酚、柚皮素、落新妇苷、绿原酸、新绿原酸及隐绿原酸在各自浓度范围内均呈现良好的线性关系，相关系数均大于0.999 5，精密度、稳定性、重复性良好，RSD<5.00%。丹参素钠、丹酚酸B、落新妇苷、绿原酸、新绿原酸及隐绿原酸平均加样回收率（n=6）为99.39%~103.43%，RSD<5%。结论：建立的HPLC-DAD法可同时测定清肝化瘀方中9种成分的含量，具有简便、快速、准确等特点。

关键词：清肝化瘀方；HPLC-DAD法；多成分测定；含量测定

Abstract：Objective: A high performance liquid chromatography-photodiode array detector (HPLC-DAD) method was used to establish a determination method for the content of multiple components of Qinggan Huayu Recipe (清肝化瘀方). Methods: Use Agela ASB C18 chromatographic column (5 μm, 4.6 mm×250 mm), use acetonitrile (A)-0.4% phosphoric acid (B), gradient elution 0 to 10 minutes, 5% to 15% A; 10 to 20 minutes, 15% to 20%A; 20 to 45 min, 20% to 30%A; 45 to 60 min, 30% to 70%A, column temperature: 25℃, flow rate 1.0 mL/min, injection volume 10 μL, detection wavelengths are 210 nm (0-11 min, detect sodium danshensu), 327 nm (11-24 min, detect chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid), 292 nm (24-35 min, detect astilbin), 286  nm (35-42 min, detect salvianolic acid B), 370 nm (42-60 min, detect quercetin, kaempferol), 290 nm (50-52.5 min, detect naringenin). Results: Sodium danshensu, salvianolic acid B, quercetin, kaempferol, naringenin, astilbin, chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid showed good linear relationships in their respective concentration ranges, with the correlation coefficients greater than 0.999 5, and the precision, stability, and reproducibility were good, with RSDs were all<5.00%. The average recoveries (n=6) of sodium danshensu, salvianolic acid B, astilbin, chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid ranged from 99.39% to 103.43%, and the RSDs were all <5%. Conclusions: The established HPLC-DAD method can simultaneously determine the contents of nine components in Qinggan Huayu Recipe, which is simple, rapid and accurate.

Key words：Qinggan Huayu Recipe; HPLC-DAD method; multicomponent determination; content determination

发布时间：2025-12-20

点击量：20