蒲黄多糖调控YAP/TAZ通路对四氯化碳诱导的小鼠肝纤维化的影响*

作者：苏志威1，薛 娟2，孙 俊2，朱 锐3，范 恒3，计春燕2

单位：1.湖北中医药大学，湖北 武汉 430065； 2.湖北中医药大学附属新华医院/湖北省中西医结合医院，湖北 武汉 430015； 3.华中科技大学附属协和医院，湖北 武汉 430022

引用：引用：苏志威，薛娟，孙俊，朱锐，范恒，计春燕.蒲黄多糖调控YAP/TAZ通路对四氯化碳诱导的小鼠肝纤维化的影响[J].中医药导报,2025,31(4):48-52.

DOI：10.13862/j.cn43-1446/r.2025.04.008

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摘要：

目的：探索蒲黄多糖对四氯化碳诱导的小鼠肝纤维化的影响。方法：将40只小鼠随机分为4组，即对照组、模型组、蒲黄多糖组、维替泊芬组。通过四氯化碳诱导小鼠建立肝纤维化模型，蒲黄多糖组小鼠每天灌胃50 mg/kg蒲黄多糖；对照组、模型组的小鼠灌胃生理盐水；维替泊芬组小鼠每天尾静脉注射5 mg/kg维替泊芬，均持续4周。通过苏木素-伊红染色法分析各组小鼠肝脏病理形态变化；通过马松染色分析各组小鼠肝脏纤维化情况；通过酶联免疫吸附测定法分析各组小鼠血清中肝功能与炎症因子相关指标的水平；通过蛋白免疫印迹分析Yes相关蛋白通路蛋白的表达。结果：模型组小鼠肝脏组织出现严重病理损伤，而蒲黄多糖组与维替泊芬组小鼠的肝脏组织损伤改善。与模型组比较，蒲黄多糖组与维替泊芬组小鼠的肝脏组织纤维化面积降低（P＜0.05）。与模型组相比，蒲黄多糖组与维替泊芬组小鼠肝功能指标改善，炎症因子水平降低（P＜0.05）；与模型组比较，蒲黄多糖组与维替泊芬组小鼠肝脏中YAP、TAZ、CTGF的表达均受到抑制（P＜0.05）。结论：蒲黄多糖可改善四氯化碳诱导的小鼠肝纤维化，该作用与蒲黄多糖调控Yes相关蛋白通路有关。

关键词：蒲黄多糖；Yes相关蛋白；肝纤维化；四氯化碳；小鼠；维替泊芬；炎症

Abstract：

Objective: To investigate the effect of Pollen typhae polysaccharide on carbon tetrachloride-induced liver fibrosis in mice. Methods: Totally 40 mice were randomly divided into 4 groups, including control group, model group, Pollen typhae polysaccharide group and verteporfin group. Carbon tetrachloride was used to induce liver fibrosis in mice. The mice were gavaged with Pollen typhae polysaccharide (50 mg/kg) per day in the Pollen typhae polysaccharide group. Mice were given normal saline by gavage in the control group and model group. Mice were given verteporfin (5 mg/kg) via tail vein every day in verteporfin group. Each group received continuous intervention for 4 weeks. Hematoxylin-eosin staining was used to analyze the pathological changes of the liver. Masson staining was used to analyze liver fibrosis in each group. The levels of liver function and inflammatory factors in serum of mice in each group were analyzed by enzyme-linked immunosorbent assay. The expression of Yes-associated protein pathway proteins was analyzed by Western blotting. Results: The liver tissue of the model group showed severe pathological damage, while the liver tissue damage of the Pollen typhae polysaccharide group and verteporfin group was improved. Compared with the model group, the area of liver fibrosis was decreased in the Pollen typhae polysaccharide group and verteporfin group (P<0.05). Compared with the model group, the liver function indexes were improved in Pollen typhae polysaccharide group and verteporfin group, and the levels of inflammatory factors were decreased (P<0.05). Compared with the model group, the expression of YAP, TAZ and CTGF in the liver was inhibited in Typha polysaccharide group and verteporfin group (P<0.05). Conclusion: Pollen typhae polysaccharide can improve carbon tetrachloride induced liver fibrosis in mice, and this process is related to the regulation of Yes-associated protein pathway by Pollen typhae polysaccharide.

Key words：Pollen typhae polysaccharide; Yes-associated protein; liver fibrosis; carbon tetrachloride; mice; verteporfin; inflammation

发布时间：2025-12-20

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