山楂酸对鼻咽癌细胞迁移与侵袭的干预作用研究*

作者：袁悦萌1，陈姝霖2，王晓娟1，王 雯1，吴仪彬1，何迎春1,3,4，周芳亮1,3,4

单位：1.湖南中医药大学医学院，湖南 长沙 410208； 2.湖南中医药大学第一中医临床学院，湖南 长沙 410208； 3.湖南中医药大学中医药防治眼耳鼻咽喉疾病湖南省重点实验室，湖南 长沙 410208； 4.湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心，湖南 长沙 410208

引用：引用：袁悦萌，陈姝霖，王晓娟，王雯，吴仪彬，何迎春，周芳亮.山楂酸对鼻咽癌细胞迁移与侵袭的干预作用研究[J].中医药导报,2025,31(4):27-33.

DOI：10.13862/j.cn43-1446/r.2025.04.005

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摘要：

目的：探讨山楂酸（MA）对鼻咽癌细胞迁移、侵袭的作用与机制。方法：将鼻咽癌细胞S18、S26分别分为药物组（10、20、30、40、50、60、70、80 μmol/L）、对照组和顺铂（CIS）组，采用噻唑蓝（MTT）比色法检测山楂酸对鼻咽癌细胞增殖能力的影响。将鼻咽癌细胞S18、S26分别分为药物组（10、15、20 μmol/L）、对照组和CIS组，采用划痕实验、Transwell侵袭小室法检测鼻咽癌细胞迁移和侵袭情况。将鼻咽癌细胞S18、S26分别分为药物组（20、40 μmol/L）、对照组和CIS组，采用蛋白免疫印迹法（Western blotting）检测细胞迁移蛋白Vimentin、N-cadherin表达水平。构建鼻咽癌细胞裸鼠足垫-腹股沟淋巴结转移模型后，将造模成功的裸鼠随机分为对照组、MA组、CIS组，每组6只。各组予相应药物干预20 d后观察裸鼠腹股沟淋巴结的大小和质量，称量肝、肾、脾质量，并评估淋巴结转移情况。结果：MTT实验显示，干预36、48 h后，药物组S18、S26细胞相对增殖率均低于对照组（P<0.05或P<0.01）。划痕实验显示，干预12、24、36、48 h后，药物组S18、S26细胞相对迁移率低于对照组（P<0.05或P<0.01）。Transwell实验显示，药物组（15、20 μmol/L）S18细胞相对侵袭率低于对照组（P<0.01）；药物组S26细胞相对侵袭率低于对照组（P<0.05）。Western blotting实验显示，药物组S18、S26细胞Vimentin、N-cadherin蛋白相对表达量低于对照组（P<0.05）。动物实验显示，MA组、CIS组裸鼠淋巴结转移率均低于对照组（P＜0.05），淋巴结、肝、脾质量均低于对照组（P＜0.05）；HE染色示MA组转移灶明显少于对照组。结论：山楂酸可抑制鼻咽癌细胞增殖、迁移及侵袭相关蛋白表达。

关键词：鼻咽癌；山楂酸；细胞迁移；细胞侵袭；上皮间质转化

Abstract：

Objective: To investigate the effects and mechanisms of maslinic acid (MA) on the migration and invasion of nasopharyngeal carcinoma (NPC) cells. Methods: NPC cell lines S18 and S26 were divided into drug groups (10, 20, 30, 40, 50, 60, 70, 80 μmol/L MA), control group, and cisplatin (CIS) group. The MTT assay was used to evaluate the inhibitory effect of MA on NPC cell proliferation. For migration and invasion assays, S18 and S26 cells were divided into drug groups (10, 15, 20 μmol/L MA), control group and CIS group. Scratch wound healing and Transwell invasion chamber assays were performed to assess cell migration and invasion. For protein analysis, S18 and S26 cells were divided into drug groups (20, 40 μmol/L MA), control group and CIS group. Western blotting was used to detect the expression levels of migration-related proteins (Vimentin, N-cadherin). A nude mouse footpad-inguinal lymph node metastasis model was established using NPC cells. Successfully modeled mice were randomly divided into control group, MA group and CIS group, 6 mice in each group. After 20 days of drug intervention, inguinal lymph node size and weight, liver/kidney/spleen weights, and lymph node metastasis were evaluated. Results: MTT assays showed that the relative proliferation rates of S18 and S26 cells in drug groups were significantly lower than those in the control group at 36 and 48 h post-treatment (P<0.05 or P<0.01). Scratch wound healing assays demonstrated that drug groups showed lower relative migration of S18 and S26 cells than control group at 12, 24, 36, and 48 h post-treatment (P<0.05 or P<0.01). Transwell assays demonstrated that drug groups (15, 20 μmol/L) showed lower relative invasion rates of S18 cells than control group (P<0.01), and that drug groups showed lower relative invasion rates of S26 cells than control group (P<0.05). Western blotting demonstrated that drug groups (S18, S26) showed lower Vimentin and N-cadherin protein expression (P<0.05). Animal experiments showed that MA group and CIS group showed lower lymph node metastasis rates than control group (P<0.05), with lower lymph node/liver/spleen weights than control group (P<0.05). MA group showed fewer metastatic foci (HE staining) than control group. Conclusion: Maslinic acid can inhibit NPC cell proliferation, migration and invasion related protein expression.

Key words：nasopharyngeal carcinoma; maslinic acid; cell migration; cell invasion; epithelial-mesenchymal transition

发布时间：2025-12-20

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