大黄䗪虫丸对宫颈癌细胞自噬与凋亡的作用及机制研究*
作者：何金秀，陈乙菲，魏 毅，于若男，姜 德
单位：新疆医科大学中医学院，新疆 乌鲁木齐 830000
引用：引用：何金秀，陈乙菲，魏毅，于若男，姜德.大黄䗪虫丸对宫颈癌细胞自噬与凋亡的作用及机制研究[J].中医药导报,2025,31(4):14-20.
DOI：10.13862/j.cn43-1446/r.2025.04.003
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摘要：
目的：探讨大黄䗪虫丸（DHZCW）对宫颈癌细胞自噬与凋亡的作用及机制。方法：通过网络药理学方法预测大黄䗪虫丸治疗宫颈癌的作用靶点和信号通路，并进行细胞实验验证，将Hela细胞按不同实验目的设空白对照组、低剂量组、中剂量组、高剂量组及顺铂组，使用CCK-8法、划痕实验、JC-1染色、流式细胞术、透射电镜观察、蛋白免疫印迹法等检测方法检测大黄䗪虫丸对宫颈癌细胞自噬与凋亡的作用及其机制。结果：（1）网络药理：大黄䗪虫丸-宫颈癌核心作用靶点90个，核心10个靶点；（2）细胞实验：与空白对照组比较，低、中、高剂量组抑制了Hela细胞的增殖活性和细胞迁移能力，能够诱导线粒体膜电位下降和促进细胞凋亡；与空白对照组比较，随着大黄䗪虫丸剂量的增加，线粒体形态显著改变，由正常的线粒体到大量肿胀，线粒体嵴减少，直至出现线粒体膜破裂和消失、线粒体空泡化和凋亡小体；与空白对照组比较，低、高剂量组Beclin1和Bcl-2蛋白表达水平下降（P＜0.05或P＜0.01），Bax蛋白表达水平升高（P＜0.01），同时低、中、高剂量组Bax/Bcl-2比值水平均高于空白对照组（P＜0.05或P＜0.01）。与空白对照组比较，低、中、高剂量组LC3B-Ⅱ/Ⅰ蛋白表达水平均升高（P＜0.05或P＜0.01）。与空白对照组比较，高剂量组cleaved Caspase-3蛋白水平升高（P＜0.01）。结论：大黄䗪虫丸可以抑制Hela细胞自噬而促进凋亡，作用效果呈剂量效应关系。
关键词：宫颈癌；Hela细胞；大黄䗪虫丸；细胞自噬；细胞凋亡
Abstract：
Objective: To investigate the mechanism of Dahuang Zhichong Wan (DHZCW) in inducing autophagy and apoptosis in cervical cancer cells. Methods: Network pharmacology was used to predict the targets and signaling pathways of DHZCW in the treatment of cervical cancer, and they were validated through cellular experiments. Hela cells were divided into blank control group, low dose group, medium dose group, high dose group and cisplatin group according to different experimental purposes. CCK-8 assay, scratch test, JC-1 staining, flow cytometry, transmission electron microscopy, and protein immunoblotting were applied to detect the effect and mechanism of DHZCW on autophagy and apoptosis of cervical cancer cells. Results: (1)Network pharmacology: DHZCW had 90 core targets in cervical cancer, with 10 core targets. (2)Cellular experiments: Compared with the blank control group, low, medium, and high dose groups inhibited the proliferation and viability of Hela cells and impaired cell migration. They could induce a decrease in mitochondrial membrane potential and promote cell apoptosis. With the increase in the dose of DHZCW, there was a significant change in the morphology of mitochondria, from normal mitochondria to large swollen ones with fewer cristae, until the appearance of broken mitochondrial membrane, vacuolization, and apoptotic bodies. Compared with the blank control group, the expression levels of Beclin1 and Bcl-2 proteins decreased in low and high dose groups (P<0.05 or P＜0.01), while the expression level of Bax protein increased (P<0.01). At the same time, low, medium and high dose groups showed higher Bax/Bcl-2 ratio than blank control group (P<0.05 or P＜0.01). Compared with the blank control group, the expression levels of LC3B-Ⅱ/Ⅰ protein increased in low, medium and high dose groups (P<0.05 or P<0.01). Compared with the blank control group, the levels of cleaved Caspase-3 protein increased in high dose group (P<0.01). Conclusion: DHZCW can inhibit autophagy in Hela cells and promote apoptosis, with a dose-dependent effect.
Key words：cervical cancer; Hela cells; Dahuang Zhechong Wan; autophagy; apoptosis
发布时间：2025-12-20
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