多重荧光定量PCR法同时定量检测金银花中山银花的研究*

作者：雷 庆，赵中开，金昊嵩

单位：自贡检验检测院，四川 自贡 643000

引用：引用：雷庆，赵中开，金昊嵩.多重荧光定量PCR法同时定量检测金银花中山银花的研究[J].中医药导报,2025,31(11):45-50.

DOI：10.13862/j.cn43-1446/r.2025.11.008

PDF： 下载PDF

摘要：目的：建立一种同时定量检测金银花中4种山银花的方法。方法：采用多重荧光定量PCR仪，分别利用金银花和山银花种源灰毡毛忍冬、红腺忍冬、华南忍冬和黄褐毛忍冬特异性核酸的引物和探针检测金银花中混合的山银花成分，通过定量标准曲线计算5种植物物种的DNA浓度。结果：采用本方法分别建立了金银花、灰毡毛忍冬、红腺忍冬、华南忍冬和黄褐毛忍冬的实时荧光定量PCR检出限和定量标准曲线。结论：该方法稳定、可靠、专属性强，能在一个实时荧光定量PCR仪反应体系中同时、快捷、准确定量地检测出金银花中4种山银花种源的DNA成分。

关键词：金银花；山银花；实时荧光定量PCR；快速检测

Abstract：

Objective: To establish a method for simultaneously quantifying four species of Lonicerae Flos in Lonicerae Japonicae Flos. Methods: Using multiplex fluorescent quantitative PCR, species-specific primers and probes for Lonicera japonica flos and four Lonicera flos species (Lonicera macranthoides, Lonicera hypoglauca, Lonicera confusa, and Lonicera fulvotomentosa) were employed to detect mixed Lonicera flos components in Lonicera japonica flos. DNA concentrations of the five plant species were calculated via quantitative standard curves. Results: Real-time fluorescent quantitative PCR detection limits and quantitative standard curves were established for Lonicera japonica flos, Lonicera macranthoides, Lonicera hypoglauca, Lonicera confusa, and Lonicera fulvotomentosa. Conclusion: This method is stable, reliable and highly specific, and it enables the simultaneous, rapid, and accurate quantitative detection of DNA from four Lonicera flos species in Lonicera japonica flos within within a single real-time fluorescent quantitative PCR reaction system.

Key words：Lonicerae japonicae flos; Lonicerae flos; real-time fluorescent quantitative PCR; rapid identification

发布时间：2025-11-29

点击量：71