鸡参芪抗癌颗粒水提物的体内外抗乳腺癌作用

作者：李 良，吴华锋，何碧钻，庞良承，覃久芸，童建军，蒋伟哲，付书婕

单位：1

引用：引用：李良，吴华锋，何碧钻，庞良承，覃久芸，童建军，蒋伟哲，付书婕.鸡参芪抗癌颗粒水提物的体内外抗乳腺癌作用[J].中医药导报,2025,31(10):8-15.

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摘要：

目的：探究鸡参芪抗癌颗粒水提物（JSQ）的抗乳腺癌作用及其机制。方法：制备不同浓度的JSQ含药培养基和条件培养基，测定药物作用24、48 h后不同给药浓度对乳腺癌细胞增殖的抑制作用，并计算IC50；检测JSQ对乳腺癌细胞凋亡的影响；通过建立乳腺癌荷瘤小鼠模型，经JSQ治疗后，检测脏器指数、抑瘤率、血清炎症因子和免疫因子以及T细胞亚群占比，观察肿瘤组织病理变化。结果：JSQ在体外实验表现出对乳腺癌细胞的显著抑制活性，且成剂量和时间依赖性，24、48 h的IC50分别为2.976、1.864 mg/mL；经JSQ诱导刺激后的Raw264.7细胞培养上清产生了对乳腺癌细胞活性更强的抑制作用，提示JSQ可能通过作用于巨噬细胞产生的某些物质增强了对肿瘤的杀伤作用；乳腺癌细胞凋亡试验结果表明，JSQ可促进乳腺癌细胞凋亡坏死。体内试验中JSQ能显著抑制乳腺癌小鼠肿瘤的生长，恢复乳腺癌小鼠的体质量，降低肿瘤生长引起的白细胞和淋巴细胞增加，减少炎症反应，提高CD8＋T细胞比例，恢复小鼠的胸腺指数，减轻肿瘤生长引起的脾脏和肝脏肿大症状，恢复其正常肝功能，同时可增加小鼠肿瘤坏死因子-α（TNF-α）、白介素-1β（IL-1β）、IL-2、IL-6、γ干扰素（INF-γ）的表达，促进肿瘤细胞的凋亡从而抑制肿瘤的生长。结论：JSQ具有一定的抗乳腺癌作用和增强免疫的作用，无明显毒副作用，对免疫系统及肝功能具有一定的保护作用，其抗肿瘤活性可能是通过免疫调节实现的。

关键词：鸡参芪抗癌颗粒；乳腺癌；小鼠；乳腺癌细胞；抗肿瘤

Abstract：

Objective: To investigate the anti-tumour activity of Ji Shenqi composite extract (JSQ) on breast cancer and its mechanism of action. Methods: Different concentrations of JSQ-containing medium and conditioned medium were prepared to determine the inhibitory effects of different administered concentrations on the proliferation of breast cancer cells after 24 h and 48h of drug action, and the IC50 was calculated. The effect of JSQ on apoptosis of breast cancer cells was detected. A hormonal mouse model of breast cancer was established, and the breast cancer mice were intervened by JSQ. The organ index, tumour suppression rate, serum inflammatory and immune factors, and percentage of T-cell subpopulations were determined. The histopathological changes of the tumour were observed after treatment with JSQ. Results: JSQ showed significant inhibitory activity against breast cancer cells in vitro, and the effect was dose-dependent and time-dependent. The IC50 of 24 h and 48 h was 2.976 mg/mL and 1.864 mg/mL respectively. Raw264.7 cell culture supernatants, induced by JSQ, produced a stronger inhibitory effect on the activity of breast cancer cells, suggesting that JSQ may enhance the tumour killing effect by acting on certain substances produced by macrophages. The results of apoptosis assay showed that JSQ promoted apoptotic necrosis of breast cancer cells. JSQ significantly inhibited the growth of tumours in mice with breast cancer in vivo, restored the body weight of mice with breast cancer, reduced the increase of leukocytes and lymphocytes induced by the growth of tumours, decreased inflammatory reactions, increased the proportion of CD8+ T cells, restored the thymic index of mice, reduced the symptoms of enlargement of the spleen and the liver induced by the growth of tumours, restored the normal liver function, increased the expression of TNF-α, IL-1β, IL-2, IL-6 and INF-γ, and promoted apoptosis of tumour cells, thus inhibiting the growth of tumours. Conclusion: JSQ has certain anti-breast cancer effects and immune-enhancing effects without obvious toxic side effects, and JSQ has certain protective effects on the immune system as well as on the liver function. Its anti-tumour activity may be achieved through immune modulation.

Key words： Objective: To investigate the anti-tumour activity of Ji Shenqi composite extract (JSQ) on breast cancer and its mechanism of action. Methods: Different concentrations of JSQ-containing medium and co

发布时间：2025-11-12

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