旋覆花地上茎叶的成分分析*

作者：张雪慧，孙孝亚，吴给给，陈随清

单位：河南中医药大学药学院，河南 郑州 450046

引用：引用：张雪慧，孙孝亚，吴给给，陈随清.旋覆花地上茎叶的成分分析[J].中医药导报,2026,32(5):77-83,104.

DOI：10.13862/j.cn43-1446/r.2026.05.012

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摘要：

目的：建立旋覆花地上茎叶的高效液相色谱（HPLC）指纹图谱，测定1-O-乙酰旋覆花内酯、1,5-O-二咖啡酰奎宁酸及无机元素含量，分析旋覆花地上茎叶成分。方法：采用Symmetry C18色谱柱（250.0 mm×4.6 mm，5.0 μm)；0.1％甲酸水溶液（A）-乙腈（B）为流动相，梯度洗脱（0~10 min，10%~15%B；10~15 min，15%~22%B；15~35 min，22%~30%B；35~45 min，30%~40%B；45~50 min，40%~50%B）；流速为1.0 mL/min；柱温30 ℃；旋覆花地上茎叶供试品溶液进样量50 μL，头状花序供试品溶液进样量10 μL；检测波长245 nm。采用中药指纹图谱相似度评价软件进行相似度分析，并对其特征性化学成分1-O-乙酰旋覆花内酯、1,5-O-二咖啡酰奎宁酸进行含量测定，同时采用电感耦合等离子体质谱法测定无机元素含量，分析地上茎叶与头状花序的成分差异。结果：建立了旋覆花地上茎叶的HPLC指纹图谱,相似度为0.984～0.996，标定共有峰27个。指认出1,5-O-二咖啡酰奎宁酸、1-O-乙酰旋覆花内酯2个共有峰，样品一致性良好。旋覆花地上茎叶共检测出23种无机元素，元素含量差异较大。结论：建立了旋覆花地上茎叶的指纹图谱，明确了旋覆花地上茎叶与头状花序的成分差异。

关键词：旋覆花；地上茎叶；指纹图谱；化学成分；含量测定；无机元素；质量分析

Abstract：

Objective: To establish the high-performance liquid chromatography (HPLC) fingerprint of the stems and leaves of Inula japonica, determine the contents of 1-O-acetylbritannilactone, 1,5-O-dicaffeoylquinic acid, and inorganic elements, and analyze the components of the aboveground stems and leaves of Inula japonica. Methods: A Symmetry C18 column (250.0 mm×4.6 mm, 5.0 μm) was used. The mobile phase consisted of 0.1% formic acid in water (A) and acetonitrile (B) with gradient elution (0-10 min, 10%-15% B; 10-15 min, 15%-22% B; 15-35 min, 22%-30% B; 35-45 min, 30%-40% B; 45-50 min, 40%-50% B). The flow rate was 1.0 mL/min, and the column temperature was 30 ℃. The injection volume was 50 μL for the test solution of the aboveground stems and leaves of Inula japonica and 10 μL for the test solution of the capitulum, and the detection wavelength was 245 nm. The similarity evaluation software of traditional Chinese medicine fingerprint was used to analyze the similarity, and the content of its characteristic chemical components 1-O-acetylinulacolactone and 1,5-O-dicaffeoylquinic acid was determined. At the same time, the content of inorganic elements was determined by inductively coupled plasma mass spectrometry, and the composition differences between the stems and leaves and the capitulum were compared and analyzed. Results: The HPLC fingerprint of the stems and leaves of Inula japonica was established. The similarity was 0.984-0.996, and 27 common peaks were calibrated. Two common peaks of 1,5-O-dicaffeoylquinic acid and 1-O-acetylbritannilactone were identified by comparison of reference substances, and the sample consistency was good. The content of inorganic elements in the stems and leaves of Inula britannica was determined. A total of 23 inorganic elements were detected, and the content of elements was quite different. Conclusion: The fingerprint of the stems and leaves of Inula japonica is established, and the difference between the stems and leaves of Inula japonica and the capitulum is clarified.

Key words：Inula japonica; aerial stems and leaves; fingerprint; chemical composition; content determination; inorganic elements; quality analysis

发布时间：2026-05-23

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