基于一测多评多指标定量联合化学计量学及CRITIC-TOPSIS分析对不同产地合欢皮质量差异评价*
作者：张 双1，魏 旭2，刘 利1，陈 飞2，魏谭军2，王春龙2，肖 成2
单位：1.达州市食品药品检验所，四川 达州 635000； 2.达州市中西医结合医院/达州市第二人民医院，四川 达州 635000
引用：引用：张双，魏旭，刘利，陈飞，魏谭军，王春龙，肖成.基于一测多评多指标定量联合化学计量学及CRITIC-TOPSIS分析对不同产地合欢皮质量差异评价[J].中医药导报,2026,32(5):69-76.
DOI：10.13862/j.cn43-1446/r.2026.05.011
PDF：
下载PDF
摘要：目的：建立不同产地合欢皮中多指标定量检测方法，结合化学计量学和CRITIC-TOPSIS法开展合欢皮质量评价研究。方法：对7省15个批次合欢皮样品进行超声提取，提取物采用一测多评（QAMS）法，以木犀草素和齐墩果酸为内参物，建立内参物与异槲皮苷、槲皮苷、槲皮素、刺五加苷E、(-)-丁香树脂酚-4-O-β-D-呋喃芹糖基-(1→2)-β-D-吡喃葡萄糖苷、(-)-丁香树脂酚-4-O-β-D-葡萄糖苷、丁香脂素、胡萝卜苷、β-谷甾醇的相对校正因子，同时采用外标法与QAMS法分别检测合欢皮中11个成分含量，验证QAMS法的可行性；根据《中华人民共和国药典》检查法测定醇溶性浸出物、总灰分和酸不溶性灰分含量。采用化学识别模式和CRITIC-TOPSIS法分析以上14个指标测量数据，建立合欢皮质量评价研究模型，对其质量差异性进行综合评价。结果：11个成分各自质量浓度范围内线性关系良好，平均加样回收率96.92%~100.11%（RSD＜2.0%），QAMS法与外标法所得各成分含量差异无统计学意义（P＞0.05）；化学计量学分析提取出2个主成分，代表14个指标87.318 5%的信息，15批样品聚为3类；槲皮苷、齐墩果酸、(-)-丁香树脂酚-4-O-β-D-呋喃芹糖基-(1→2)-β-D-吡喃葡萄糖苷、β-谷甾醇和槲皮素的VIP值＞1；CRITIC-TOPSIS法分析结果显示15批合欢皮中S6的质量最优（Ci值为0.606 6）。结论：建立的多指标定量检测方法简便、准确，结合化学计量学及CRITIC-TOPSIS法可为合欢皮质量控制与评价提供参考。
关键词：合欢皮；一测多评法；化学计量学；CRITIC-TOPSIS法；质量评价
Abstract：
Objective: To establish a multi-index quantitative detection for Albiziae cortex from different origins and to evaluate its quality by combining chemometrics with the CRITIC-TOPSIS method. Methods: The samples of Albiziae cortex from 15 batches in 7 provinces were extracted by ultrasonic treatment, and the extracts were detected by Quantitative analysis of multi-components by single marker (QAMS). Luteolin and oleanolic acid served as the internalreference, and a multi-point calibration method was utilized to calculate the relative correction factors for isoquercitrin, quercitrin, quercetin, eleutheroside E, (-)-syringaresinol-4-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside, episyringaresinol 4'-O-β-D-glncopyranoside, syringaresinol, daucosterol, β-sitosterol to be tested. The contents of 11 components in Albiziae cortex were determined by both the external standard method and the QAMS method to verify the feasibility of the QAMS method. The contents of alcohol-soluble extract, total ash and acid-insoluble ash were determined according to the Chinese Pharmacopoeia. The chemical identification model and CRITIC-TOPSIS method were used to analyze the measurement data of the above 14 indicators, and the quality evaluation research model of Albiziae cortex was established to comprehensively evaluate the quality differences. Results: The linear relationship was good in the range of each mass concentration of 11 components in Albiziae cortex, with the average recovery rate of 96.92%-100.11% (RSD<2.0%), and there was no significant difference in the content of each component between QAMS method and the external standard method (P>0.05). Chemometrics analysis extracted 2 principal components, which represent 87.318 5% information of 14 indicators. The 15 batches of samples were clustered into 3 categories. The VIP values of quercitrin, oleanolic acid, (-)-syringaresinol-4-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside, β-sitosterol and quercetin were>1. The results of CRITIC-TOPSIS analysis showed that the quality of S6 in 15 batches of Albiziae cortex was the best (Ci value was 0.606 6). Conclusion: The established multi-index quantitative detection is simple and accurate. Combined with chemometrics and CRITIC-TOPSIS method, it can provide reference for quality control and evaluation of Albiziae cortex.
Key words：Albiziae cortex; Quantitative analysis of multi-components by single marker; chemometrics; CRITIC-TOPSIS; quality evaluation
发布时间：2026-05-23
点击量：407