芍药汤中白芍关键成分的指纹图谱和网络药理学研究*

作者：李高天1，肖海龙1，杨 直1，林 捷1，阮家钊2

单位：1.杭州市食品药品检验科学研究院，浙江 杭州 310022； 2.浙江中医药大学基础医学院，浙江 杭州 310053

引用：引用：李高天，肖海龙，杨直，林捷，阮家钊.芍药汤中白芍关键成分的指纹图谱和网络药理学研究[J].中医药导报,2026,32(5):55-60.

DOI：10.13862/j.cn43-1446/r.2026.05.009

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摘要：

目的：联合HPLC指纹图谱及网络药理学技术预测芍药汤中源于白芍的关键成分，并探讨其关键成分含量变化与芍药汤配伍增效原理的关系。方法：采用Waters SymmetryShieldTM RP18（4.6 mm×250.0 mm，5.0 μm）色谱柱，以乙腈-0.1%甲酸水为流动相，梯度洗脱，检测波长为254 nm，柱温为30 ℃，进样量为10 μL，建立芍药汤及白芍水煎液HPLC指纹图谱，指认源于白芍的色谱峰；通过PPI网络中化合物与靶点蛋白、信号通路二者的连接度筛选出方中白芍的关键成分，并采用外标法对其进行含量测定。结果：10批白芍水煎液和10批芍药汤的指纹图谱相似度均>0.90，经对照品指认后确认6个色谱峰；指认的色谱峰具有广泛的靶点和网络通路，其中芍药内酯苷、芍药苷、苯甲酰芍药苷与白芍的主要药理作用密切相关。与白芍水煎液相比，芍药汤中芍药苷的含量有所提高，但芍药内酯苷和苯甲酰芍药苷的含量却有不同程度的下降。结论：所建立的指纹图谱方法准确、可靠；芍药汤中白芍配伍后增效的物质基础可能主要与芍药苷有关，从而阐明了芍药汤配伍的合理性和科学性。

关键词：芍药汤；白芍；关键成分；指纹图谱；网络药理学；配伍

Abstract：

Objective: To predict the key components derived from Paeoniae Radix Alba in Shaoyao decoction (SYD) by combining HPLC fingerprinting and network pharmacology, and to explore the relationship between the content changes of these key components and the synergistic principle of SYD through its formulation compatibility. Methods: The Waters SymmetriyShieldTM RP18 (4.6 mm×250.0 mm, 5.0 μm) was used, with acetonitrile-0.1% formic acid in water as the mobile phase under gradient elution. The detection wavelength was set at 254 nm, with the column temperature at 30 ℃, and the injection volume was 10 μL. The HPLC fingerprints of SYD and the water decoction of Paeoniae Radix Alba were established, and the chromatographic peaks derived from Paeoniae Radix Alba were identified. The key components of Paeoniae Radix Alba in the formula were screened based on their degree of connectivity with target proteins and signaling pathways in the PPI network, and their contents were determined using the external standard method. Results: The fingerprint similarities of 10 batches of Paeoniae Radix Alba water decoction and 10 batches of SYD were all>0.90, and 6 chromatographic peaks were confirmed after identification of the reference substance. The identified chromatographic peaks cover a wide range of targets and network pathways. Among them, albiflorin, paeoniflorin, and benzoylpaeoniflorin are closely associated with the main pharmacological effects of Paeoniae Radix Alba. The content of paeoniflorin in SYD is higher than that in the corresponding water decoction of Paeoniae Radix Alba, while the contents of albiflorin and benzoylpaeoniflorin are relatively decreased. Conclusion: The established fingerprint method is accurate and reliable. The material basis for the enhanced efficacy of Paeoniae Radix Alba in Shaoyao Decoction through compatibility is likely underpinned mainly by paeoniflorin, which sheds light on the scientific rationale behind the formulation's compatibility.

Key words：Shaoyao decoction; Paeoniae Radix Alba; key components; fingerprint; network pharmacology; compatibility

发布时间：2026-05-23

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