千里光总黄酮响应面法提取优化与抗氧化活性研究*

作者：罗春燕1,2,3，林锦霞3,4，许钧西1,2，林淑贞1,2，陈艳锦1,2，张 宽1,2，林诗莹5

单位：1.福建中医药大学附属第二人民医院，福建 福州 350001； 2.福建省医疗机构中药制剂重点实验室（福建中医药大学），福建 福州 350001； 3.道地药材品质保障与资源持续利用全国重点实验室，北京 100700； 4.福建中医药大学附属第三人民医院，福建 福州 350108； 5.福建中医药大学，福建 福州 350108

引用：引用：罗春燕，林锦霞，许钧西，林淑贞，陈艳锦，张宽，林诗莹.千里光总黄酮响应面法提取优化与抗氧化活性研究[J].中医药导报,2026,32(3):44-50.

DOI：10.13862/j.cn43-1446/r.2026.03.008

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摘要：

目的：研究千里光总黄酮提取工艺的优化，并测定其抗氧化性。方法：基于单因素试验结合响应面法优化千里光总黄酮的提取工艺，并评估其体外抗氧化能力。结果：溶剂体系选用52%乙醇溶液，料液比控制在1∶47（g/mL），提取温度在79 ℃条件下进行41 min水浴提取。经验证实验测得总黄酮提取达53.27 mg/g（以原料干质量计），该工艺参数显著提高了黄酮类物质的溶出效率。抗氧化试验中千里光总黄酮在DPPH和ABTS自由基清除中IC50分别为0.40 mg/mL和3.76 mg/mL。结论：响应面法优化千里光总黄酮提取工艺方案设计稳定可行，且千里光具有良好的抗氧化能力。

关键词：千里光；总黄酮；响应面法；抗氧化；工艺优化

Abstract：

Objective: To optimize the extraction process of total flavonoids from Senecio scandens and determine its antioxidant activity. Methods: Based on single factor and response surface analysis, the extraction process of total flavonoids from Senecio scandens was optimized, and its antioxidant activity was investigated. Results: The optimum extraction process of total flavonoids from Senecio scandens was as follows: the volume fraction of ethanol was 52%, the ratio of material to liquid was 1∶47 (g/mL), the extraction temperature was 79 ℃, and the extraction time was 41 min. The extraction rate of total flavonoids from Senecio scandens was 53.27 mg/g, which was close to the predicted value. The IC50 of total flavonoids in DPPH and ABTS free radical scavenging were 0.40 mg/mL and 3.76 mg/mL, respectively. Conclusion: The design of the extraction process of total flavonoids from Senecio scandens based on response surface optimization was stable and feasible, and Senecio scandens had good antioxidant capacity.

Key words：Senecio scandens Buch.-Ham; total flavonoids; response surface method; antioxidant; process optimization.

发布时间：2026-03-28

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