基于HPLC-MS/MS技术的蒙药健脾五味丸体外化学成分及血中移行成分研究*

作者：张玉婷1，莎日娜2，何春龙1，王焕芸1，范 蕾1，孙丽君1

单位：1.内蒙古医科大学药学院，内蒙古 呼和浩特 010107； 2.内蒙古医科大学第二附属医院，内蒙古 呼和浩特 010110

引用：引用：张玉婷，莎日娜，何春龙，王焕芸，范蕾，孙丽君.基于HPLC-MS/MS技术的蒙药健脾五味丸体外化学成分及血中移行成分研究[J].中医药导报,2025,31(5):71-79.

DOI：10.13862/j.cn43-1446/r.2025.05.012

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摘要：

目的：通过测定蒙药健脾五味丸体外及小鼠灌胃后血中移行成分，研究其主要药效物质基础。方法：采用高效液相色谱-串联质谱（HPLC-MS/MS）技术，SepaxHP-C18柱（4.6 mm×250 mm，5 μm），流动相为1%甲酸（A）-乙腈（B）梯度洗脱，将混合对照品溶液、健脾五味丸供试品溶液、空白血清和不同时间段的含药血清进行定性分析，使用全扫描模式正、负离子检测模式下采集色谱图，通过比对Xcalibur 3.0软件数据库、文献以及部分对照品的离子流图、质谱碎片裂解信息，鉴定目标化合物。结果：健脾五味丸供试品溶液鉴定出47个化合物，包括黄酮类、二萜类、环烯醚萜类和酚酸类，共鉴定出42个入血成分，其中30 min入血成分有40个，60 min入血成分有38个，90 min入血成分有37个，120 min入血代谢产物有32个。结论：该方法灵敏度高，可为蒙药健脾五味丸的药效物质基础研究提供参考。

关键词：健脾五味丸；蒙药；高效液相色谱-串联质谱；化学成分；移行成分；小鼠

Abstract：

Objective: To investigate the pharmacodynamic material basis of Mongolian medicine Jianpi Wuwei Pill by analyzing its in vitro chemical components and blood-transported components after intragastric administration in mice. Methods: HPLC-MS/MS was performed on a Sepax HP-C18 column (4.6 mm×250 mm, 5 μm) with a mobile phase of 1% formic acid (A)-acetonitrile (B) under gradient elution. Qualitative analysis was conducted on mixed reference solutions, Jianpi Wuwei Pill test solutions, blank serum, and drug-containing serum collected at different time points. Chromatograms were acquired in full scan mode under both positive and negative ion detection. Target compounds were identified by comparing retention times, ion chromatograms, mass spectral fragmentation patterns, and literature data using the Xcalibur 3.0 software database. Results: A total of 47 compounds were identified in the test solution, including flavonoids, diterpenoids, iridoids, and phenolic acids. Forty-two blood-transported components were detected, with 40, 38, 37, and 32 components observed at 30, 60, 90, and 120 min post-administration, respectively. Conclusion: The established method exhibits high sensitivity and provides a reference for elucidating the pharmacodynamic material basis of Jianpi Wuwei Pill.

Key words：Jianpi Wuwei Pill; Mongolian medicine; HPLC-MS/MS; chemical components; migratory components; mice

发布时间：2025-12-31

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