基于Wnt/β-catenin信号通路探讨加味肺复方对肺腺癌A549细胞荷瘤裸鼠的影响*

作者：胡玉田1，潘 博2，傅剑锋2，潘敏求2

单位：1.湖南中医药大学第一附属医院，湖南 长沙 410007； 2.湖南省中西医结合医院，湖南 长沙 410006

引用：引用：胡玉田，潘博，傅剑锋，潘敏求.基于Wnt/β-catenin信号通路探讨加味肺复方对肺腺癌A549细胞荷瘤裸鼠的影响[J].中医药导报,2025,31(12):8-13,74.

DOI：10.13862/j.cn43-1446/r.2025.12.002

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摘要：目的：探讨加味肺复方对肺腺癌A549细胞荷瘤裸鼠瘤体及Wnt/β-连环蛋白（β-catenin）信号通路的影响。方法：从65只BALB/c-nu裸鼠中随机抽取10只作为空白对照组，剩余55只裸鼠右前肢腋下接种人肺腺癌系A549细胞，建立荷瘤小鼠模型。将50只荷瘤小鼠模型随机分为模型组（蒸馏水）、加味肺复方低剂量组、加味肺复方中剂量组、加味肺复方高剂量组及阳性组（顺铂），每组10只。测量瘤体体积和瘤体质量。采用蛋白质印迹法（Western blotting）检测裸鼠瘤体组织β-catenin表达水平；采用实时荧光定量PCR（qPCR）检测裸鼠瘤体组织髓细胞增生原癌基因（c-Myc） mRNA、c-jun mRNA、细胞周期素D1（CyclinD1） mRNA表达水平。结果：加味肺复方中剂量组、加味肺复方高剂量组及阳性组裸鼠瘤体体积及瘤体质量均低于模型组（P＜0.01或P＜0.05）；加味肺复方低剂量组裸鼠瘤体体积及瘤体质量与模型组比较，差异无统计学意义（P＞0.05）。加味肺复方高剂量组、阳性组裸鼠瘤体组织c-Myc mRNA、c-jun mRNA、Cyclin D1 mRNA相对表达量均低于模型组（P＜0.01或P＜0.05）；加味肺复方中、低剂量组裸鼠瘤体组织c-Myc mRNA、c-jun mRNA、Cyclin D1 mRNA相对表达量与模型组比较，差异无统计学意义（P＞0.05）。加味肺复方低剂量组、加味肺复方中剂量组、加味肺复方高剂量组及阳性组裸鼠β-catenin蛋白相对表达量均低于模型组（P＜0.01或P＜0.05）。结论：加味肺复方可抑制肺腺癌A549细胞荷瘤裸鼠瘤体生长，且抑瘤效果具有浓度依赖性，其机制可能与调节Wnt/β-catenin信号通路有关。

关键词：肺腺癌；加味肺复方；A549细胞；Wnt/β-catenin信号通路；裸鼠

Abstract：

Objective: To investigate the effects of Jiawei Feifu formula on tumor growth and the Wnt/β-catenin signaling pathway in nude mice bearing lung adenocarcinoma A549 cells. Methods: Totally 65 BALB/c-nu nude mice were used in the study, and 10 mice were randomly selected as the blank control group. The remaining 55 mice were inoculated with the human lung adenocarcinoma A549 cells subcutaneously in the right axillary fossa to establish the tumor-bearing mouse model. The 50 BALB/c-nu mice were divided into model group (distilled water), Jiawei Feifu formula low dose group, Jiawei Feifu formula medium dose group, Jiawei Feifu formula high dose group, and positive control group (cisplatin), 10 mice in each. The tumor volume and weight were assessed. Western blotting (WB) was employed to detect the expression levels of β-catenin nuclear protein. Quantitative real-time PCR (qPCR) was used to detect the expression levels of c-Myc mRNA, c-jun mRNA, and CyclinD1 mRNA. Results: The Jiawei Feifu formula medium dose group, Jiawei Feifu formula high dose group, and positive control group showed lower tumor volume and tumor weight than model group (P<0.01 or P<0.05). No statistically significant difference was observed in tumor volume or tumor mass between the Jiawei Feifu formula low dose group and model group in nude mice (P>0.05). The Jiawei Feifu formula high dose group, and positive control group showed lower relative expression levels of c-Myc mRNA, c-jun mRNA and Cyclin D1 mRNA than model group (P<0.01 or P<0.05). Compared with the model group, there were no statistically significant differences in the relative expression levels of c-Myc mRNA, c-jun mRNA and Cyclin D1 mRNA in the Jiawei Feifu formula low dose group, and Jiawei Feifu formula medium dose group (P>0.05). The Jiawei Feifu formula low dose group, Jiawei Feifu formula medium dose group, Jiawei Feifu formula high dose group, and positive control group showed lower relative expression levels of β-catenin than model group (P<0.01 or P<0.05). Conclusion: Jiawei Feifu formula can inhibit tumor growth in mice bearing lung adenocarcinoma A549 cells, and the tumor-inhibiting effect is concentration-dependent. Its mechanism may be related to the regulation of the Wnt/β-catenin signaling pathway.

Key words：lung adenocarcinoma; Jiawei Feifu formula; A549 cells; Wnt/β-catenin; signaling pathway; nude mice

发布时间：2025-12-31

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