益气养血方通过激活CD4+T细胞抑制食管癌增殖的机制研究*

作者：轩艳红，张洪亮，王 月，张立平

单位：新疆医科大学第四附属医院/新疆医科大学第四临床医学院，新疆 乌鲁木齐 830000

引用：引用：轩艳红，张洪亮，王月，张立平.益气养血方通过激活CD4+T细胞抑制食管癌增殖的机制研究[J].中医药导报,2025,31(5):33-37.

DOI：10.13862/j.cn43-1446/r.2025.05.006

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摘要：目的：探讨益气养血方通过激活CD4+T淋巴细胞杀伤作用抑制食管癌细胞增殖的机制。方法：采用ECA109人食管癌细胞和CD4+T淋巴细胞共培养模型，加入0.5 μg/mL益气养血方进行干预，通过细胞计数（CCK-8）实验、流式细胞术、酶联免疫吸附试验（ELISA）和免疫印迹（Western blotting）等方法，观察益气养血方对食管癌细胞增殖、凋亡及磷脂酰肌醇-3-激酶/蛋白激酶B（PI3K/Akt）信号通路的影响。结果：CCK-8和流式细胞术表明益气养血方能进一步增强CD4+T细胞的杀伤作用，显著降低食管癌细胞的存活率（P<0.05）。ELISA结果显示，在共培养体系中益气养血方能够显著上调γ干扰素（IFN-γ）、肿瘤坏死因子-α（TNF-α）和乳酸脱氢酶（LDH）的表达水平（P<0.05）。Western blotting结果显示，在共培养体系中益气养血方能够显著下调食管癌细胞中p-PI3K和p-Akt1的表达水平，抑制PI3K/Akt信号通路的激活（P<0.05）。结论：益气养血方能通过激活CD4+T淋巴细胞的杀伤作用，抑制食管癌细胞增殖，其机制可能与抑制PI3K/Akt信号通路的激活有关。

关键词：食管癌；益气养血方；CD4+T细胞；细胞增殖；PI3K/Akt信号通路

Abstract：

Objective: To explore the mechanism of Yiqi Yangxue decoction inhibiting the proliferation of esophageal cancer cells by activating the killing effect of CD4+T lymphocytes. Methods: The co-culture model of ECA109 human esophageal cancer cells and CD4+T lymphocytes was used, and Yiqi Yangxue decoction (0.5 μg/mL) was added for intervention. Through cell counting kit-8 (CCK-8) experiments, flow cytometry, enzyme-linked immunosorbent assay (ELISA), and Western blotting, the effects of Yiqi Yangxue decoction on esophageal cancer cell proliferation, apoptosis, and the phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) signaling pathway were observed. Results: CCK-8 and flow cytometry showed that Yiqi Yangxue decoction could further enhance the killing effect of CD4+T cells and significantly reduce the survival rate of esophageal cancer cells (P<0.05). ELISA results showed that Yiqi Yangxue decoction could significantly up-regulate the expression levels of IFN-γ, TNF-α, and LDH in the co-culture system (P<0.05). Western blotting results showed that Yiqi Yangxue decoction could significantly down-regulate the expression levels of p-PI3K and p-Akt1 in esophageal cancer cells in the co-culture system, and inhibit the activation of the PI3K/Akt signaling pathway (P<0.05). Conclusion: Yiqi Yangxue decoction can inhibit the proliferation of esophageal cancer cells by activating the killing effect of CD4+T lymphocytes, and its mechanism may be related to the inhibition of the activation of the PI3K/Akt signaling pathway.

Key words：esophageal cancer; Yiqi Yangxue decoction; CD4+T cells; cell proliferation; PI3K/Akt signaling pathway

发布时间：2025-12-30

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