牛大力总生物碱的抗肝癌作用及其作用机制*
作者：莫惠宁1,2，裴京楠2，李 倩2，韦彩柳2,3，张洪平2,4
单位：1.南宁市第一人民医院，广西 南宁 530200； 2.柳州市中药（壮瑶药）制剂研发重点实验室，广西 柳州 545000； 3.柳州市工人医院，广西 柳州 545000； 4.柳州市中医医院，广西 柳州 545000
引用：引用：莫惠宁，裴京楠，李倩，韦彩柳，张洪平.牛大力总生物碱的抗肝癌作用及其作用机制[J].中医药导报,2025,31(5):28-32.
DOI：10.13862/j.cn43-1446/r.2025.05.005
PDF：
下载PDF
摘要：
目的：探究牛大力总生物碱（AMC）的抗肿瘤活性及其作用机制。方法：利用有机溶剂提取法制备AMC，MTT法检测AMC对肝癌HepG2细胞和MHCC97H细胞增殖能力的影响，划痕实验和Transwell实验检测AMC对HepG2细胞迁移和侵袭能力的影响；为了探究AMC对肿瘤生长的影响，用HepG2和BALB/c-nu小鼠建立裸鼠移植瘤模型。结果：与对照组比较，AMC对HepG2细胞、MHCC97H的增殖有不同程度的抑制作用，且呈剂量依赖性，其增殖率差异有统计学意义（P＜0.01）。AMC对于HepG2细胞和MHCC97H细胞的半数抑制质量浓度（IC50）分别为1 005.0、832.8 μg/mL。与对照组比较，AMC组的细胞迁移、侵袭能力均减弱。牛大力总生物碱组小鼠肿瘤体积和质量明显减小。结论：牛大力总生物碱可抑制肝癌细胞的增殖，亦可抑制HepG2细胞的迁移和侵袭；牛大力总生物碱对BALB/c-nu荷瘤小鼠的肿瘤有抑制作用，其作用机制可能与其抑制肿瘤细胞的增殖、侵袭和迁移有关。
关键词：肝癌；牛大力；总生物碱；抗肿瘤活性；侵袭转移；HepG2细胞；MHCC97H细胞；小鼠
Abstract：
Objective: To study the anti-tumor effect and mechanism of total alkaloids of Millettia speciosa Champ (AMC). Methods: Organic solvent extraction method was used to prepare AMC. The proliferation of HepG2 and MHCC97H cells was detected by MTT assay. The migration and invasion abilities of HepG2 cells were analyzed by Wound healing assay and Transwell test. To explore the effect of AMC on tumor growth, HepG2 cell and BALB/c-nu mice were used to establish mouse models of transplanted tumors. Results: Compared with the control group, the proliferation of HepG2 cells and MHCC97H cells were inhibited by AMC in a dose-dependent manner (P<0.01). The IC50 of AMC for HepG2 cells and MHCC97H cells were 1 005.0 and 832.8 μg/mL, respectively. Compared with the control group, the cell migration and invasion of hepG2 cell were decreased by AMC. The tumor volume and weight were reduced in BALB/c-nu mice by AMC respectively. Conclusion: The proliferation, migration and invasion of HepG2 cells were inhibited by AMC, respectively. The tumor of BALB/c-nu mice may be inhibited by AMC through inhibiting proliferation, migration and invasion of tumor.
Key words：hepatocarcinoma; Millettia speciosa Champ; total alkaloids; anti-tumouractivity; invasion and metastasis; HepG2 cells; MHCC97H cells; mice
发布时间：2025-12-30
点击量：5