疏调健运消痤饮调控PI3K/Akt信号通路对胰岛素样生长因子1诱导SZ95皮脂腺细胞脂质分泌的影响*

作者：翟曼吟1，邵梦秋1，张婧琰1，赵贵霜1，刘 娟1，李孟瑶1，姜丽娟2

单位：1.云南中医药大学第一临床医学院，云南 昆明 650500； 2.云南中医药大学第一附属医院，云南 昆明 650000

引用：引用：翟曼吟，邵梦秋，张婧琰，赵贵霜，刘娟，李孟瑶，姜丽娟.疏调健运消痤饮调控PI3K/Akt信号通路对胰岛素样生长因子1诱导SZ95皮脂腺细胞脂质分泌的影响[J].中医药导报,2025,31(5):13-19.

DOI：10.13862/j.cn43-1446/r.2025.05.003

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摘要：

目的：探讨疏调健运消痤饮对胰岛素样生长因子-1（IGF-1）诱导的SZ95皮脂腺细胞痤疮模型皮脂生成与分泌的影响及潜在机制。方法：利用70 ng/mL IGF-1诱导48 h，建立SZ95皮脂腺细胞体外痤疮模型。将SZ95皮脂腺细胞分为对照组、模型组及疏调健运消痤饮组（20、50、100、150 μg/mL）。对照组SZ95皮脂腺细胞采用基础培养条件；模型组SZ95皮脂腺细胞采用IGF-1（70 ng/mL）进行诱导刺激48 h；疏调健运消痤饮组SZ95皮脂腺细胞在IGF-1（70 ng/mL）处理48 h后，分别更换为20、50、100、150 μg/mL的疏调健运消痤饮的含药培养基干预48 h。采用噻唑蓝（MTT）检测细胞活力，油红O染色观察并定量检测SZ95皮脂腺细胞皮脂分泌水平，q-PCR法检测SZ95皮脂腺细胞IGF-1 mRNA、IGF-1受体（IGF-1R） mRNA、叉头框蛋白O1（FoxO1） mRNA表达水平，Western blotting检测SZ95皮脂腺细胞IGF-1、IGF-1R、蛋白激酶（Akt）、磷脂酰肌醇3激酶（PI3K）、胆固醇调节元件结合蛋白（SREBP1）、雄激素受体（AR）及FoxO1蛋白表达水平。结果：MTT实验表明，模型组细胞存活率低于对照组（P＜0.05）；疏调健运消痤饮组细胞存活率低于模型组（P＜0.01）。油红染色O实验显示，模型组脂滴相对面积高于对照组（P＜0.01）；疏调健运消痤饮组脂滴相对面积低于模型组（P＜0.01）。q-PCR结果显示，模型组SZ95皮脂腺细胞IGF-1 mRNA及IGF-1R mRNA相对表达量高于对照组（P＜0.01），FoxO1 mRNA相对表达量低于对照组（P＜0.01）；疏调健运消痤饮组SZ95皮脂腺细胞IGF-1 mRNA及IGF-1R mRNA相对表达量均低于模型组（P＜0.01），FoxO1 mRNA相对表达量高于模型组（P＜0.05或P＜0.01）。Western blotting结果表明，模型组SZ95皮脂腺细胞IGF-1、IGF-1R、Akt、PI3K、SREBP1及AR蛋白相对表达量高于对照组（P＜0.01）；疏调健运消痤饮组（20、50、100、150 μg/mL）SZ95皮脂腺细胞IGF-1R及Akt蛋白相对表达量均低于模型组（P＜0.05或P＜0.01）；疏调健运消痤饮组（50、100、150 μg/mL）SZ95皮脂腺细胞IGF-1、PI3K及AR蛋白相对表达量均低于模型组（P＜0.05或P＜0.01）；疏调健运消痤饮组（100、150 μg/mL）SZ95皮脂腺细胞SREBP1蛋白相对表达量低于模型组（P＜0.01）。模型组SZ95皮脂腺细胞FoxO1蛋白相对表达量低于对照组（P＜0.05）；疏调健运消痤饮组（50、100 150 μg/mL）SZ95皮脂腺细胞FoxO1蛋白相对表达量均高于模型组（P＜0.05或P＜0.01）。结论：疏调健运消痤饮可通过抑制IGF-1诱导的PI3K/Akt通路的激活，增强FoxO1转录，降低成脂相关因子IGF-1、SREBP1、AR的表达，从而减少皮脂腺细胞的脂质分泌，以防治痤疮。

关键词：痤疮；疏调健运消痤饮；SZ95皮脂腺细胞；PI3K/Akt信号通路；脂质代谢；胰岛素样生长因子-1

Abstract：

Objective: To explore the effect and potential mechanism of Shutiao Jianyun Xiaocuo decoction on sebaceous production and secretion in IGF-1 induced sebaceous gland cell acne model. Methods: SZ95 sebaceous gland cells were induced with 70 ng/mL insulin-like growth factor-1 (IGF-1) for 48 h to establish an in vitro acne model. SZ95 sebaceous gland cells were divided into control group, model group and Shutiao Jianyun Xiaocuo decoction group (20, 50, 100 and 150 μg/mL). The SZ95 cells were cultured under standard conditions in control group. The SZ95 sebaceous gland cells in the model group were stimulated with IGF-1 at a concentration of 70 ng/mL for 48 hours. The SZ95 sebaceous gland cells in the Shutiao Jianyun Xiaocuo decoction group were treated with IGF-1 at a concentration of 70 ng/mL for 48 hours, followed by replacement with medium containing Shutiao Jianyun Xiaocuo decoction at concentrations of 20, 50, 100 and 150 μg/mL for an additional 48 hours. Cell viability was assessed using the MTT assay, and Oil Red O staining was performed to observe and quantify sebum secretion levels. The expression levels of IGF-1 mRNA, insulin-like growth factor 1 receptor (IGF-1R) mRNA, and forkhead box protein O1 (FoxO1) mRNA were measured by q-PCR. Western blotting analysis was conducted to determine the protein expression levels of lipid-related factors such as IGF-1, IGF-1R, protein kinase B (Akt), phosphatidylinositol 3 kinase (PI3K), sterol regulatory element-binding protein 1 (SREBP1), androgen receptor (AR), and FoxO1. Results: MTT assay showed that the cell survival rate of the model group was lower than that of the control group (P<0.05), and that the Shutiao Jianyun Xiaocuo decoction group showed lower cell survival rate than model group (P<0.01). Oil red staining O test showed that the relative area of lipid droplets in the model group was higher than that in the control group (P<0.01), and that the Shutiao Jianyun Xiaocuo decoction group showed lower relative area of lipid droplets than model group (P<0.01). According to the results of q-PCR, the model group showed higher relative expression of IGF-1 mRNA and IGF-1R mRNA than control group (P<0.01), while lower relative expression of FoxO1 mRNA than control group (P<0.01). The Shutiao Jianyun Xiaocuo decoction group showed lower relative expression of IGF-1 mRNA and IGF-1R mRNA than model group (P<0.01), while higher relative expression of FoxO1 mRNA than model group (P<0.05 or P<0.01). Western blotting results showed that the relative expressions of IGF-1, IGF-1R, Akt, PI3K, SREBP1 and AR proteins in model group were higher than those in control group (P<0.01), and that the Shutiao Jianyu Xiaocuo decoction group (20, 50, 100 and 150 μg/mL) showed lower relative expressions of IGF-1-R and Akt protein than model group (P<0.05 or P<0.01). The Shutiao Jianyun Xiaocuo decoction group (50, 100 and 150 μg/mL) showed lower relative expressions of IGF-1, PI3K and AR protein than model group (P<0.05 or P<0.01). The Shutiao Jianyu Xiaocuo decoction group (100, 150 μg/mL) showed lower relative expression of SREBP-1 protein than model group (P<0.01). The model group showed lower relative expression of FoxO1 protein than control group (P<0.05). The Shutiao Jianyu Xiaocuo decoction group (50 100 and 150 μg/mL) showed higher relative expression of FoxO1 protein than model group (P<0.05 or P<0.01). Conclusions: Shutiao Jianyu Xiaocuo decoction can inhibit the IGF-1-induced activation of PI3K/Akt signaling pathway, enhance the transcription of FoxO1, and reduce the expression of adipogenic factors IGF-1, SREBP1 and AR, thereby reducing lipid secretion of sebocytes to prevent and treat acne.

Key words：acne; Shutiao Jianyun Xiaocuo decoction; SZ95 sebaceous gland cells; PI3k/Akt signaling pathway; lipid metabolism; insulin-like growth factor-1

发布时间：2025-12-23

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