天马颗粒剂基于miR-34a调控大肠癌细胞增殖及凋亡的实验研究*

作者：马亚波1，胡响当2，鲁海燕2，罗 敏2，徐胜艳2，李磊磊2，杨宗亮2

单位：1.湖南中医药大学，湖南 长沙 410208；2.湖南中医药大学第二附属医院，湖南 长沙 410005

引用：引用：马亚波，胡响当，鲁海燕，罗敏，徐胜艳，李磊磊，杨宗亮.天马颗粒剂基于miR-34a调控大肠癌细胞增殖及凋亡的实验研究[J].中医药导报,2025,31(4):21-26.

DOI：10.13862/j.cn43-1446/r.2025.04.004

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摘要：

目的：探究天马颗粒剂基于miR-34a调控大肠癌细胞增殖及凋亡的分子机制。方法：采用不同浓度的天马颗粒剂含药血清孵育SW480细胞，24 h后通过MTT法筛选天马颗粒剂含药血清作用浓度；将SW480细胞分为正常对照组、空白血清组、正常剂量组、50%正常剂量组。正常对照组正常培养，正常剂量组予17.0 μg/mL天马颗粒剂含药血清干预，50%正常剂量组予8.5 μg/mL天马颗粒剂含药血清干预，空白血清组予空白血清干预，24 h后采用RT-PCR检测miR-34a表达，采用MTT检测细胞增殖，采用流式细胞术检测细胞的凋亡情况，采用Western blotting检测细胞中p53及cleaved Caspase-3表达。将SW480细胞分为天马颗粒剂组、天马颗粒剂+NC inhibitor组、天马颗粒剂+miR-34a inhibitor组。天马颗粒剂组予天马颗粒剂含药血清（17.0  μg/mL）干预，天马颗粒剂+NC inhibitor组及天马颗粒剂+miR-34a inhibitor组分别给予NC inhibitor及miR-34a inhibitor转染，随后予天马颗粒剂含药血清（17.0 μg/mL）干预，24 h后采用Western blotting检测细胞中p53及cleaved Caspase-3的表达。结果：天马颗粒剂含药血清体外作用浓度为17.0 μg/mL。天马颗粒剂可抑制SW480细胞的增殖，促进SW480细胞的凋亡，上调大肠癌细胞中miR-34a、p53、cleaved Caspase-3的表达，且对大肠癌细胞的调控作用具有剂量依赖性。抑制miR-34a可逆转天马颗粒对大肠癌细胞的促凋亡及抗增殖作用。结论：天马颗粒剂可通过上调miR-34a促进p53、cleaved Caspase-3的表达抑制大肠癌细胞增殖，促进大肠癌细胞凋亡。

关键词：大肠癌；天马颗粒剂；miR-34a；细胞增殖；细胞凋亡

Abstract：

Objective: To investigate the molecular mechanism of Tianma granules in regulating the proliferation and apoptosis of colorectal cancer cells based on miR-34a. Methods: SW480 cells were incubated with drug-containing serum of Tianma granules at different concentrations. After 24 hours, MTT assay was used to screen the action concentration of drug-containing serum of Tianma granules. SW480 cells were divided into normal control group, blank serum group, normal dose group and 50% normal dose group. The SW480 cells were cultured normally in normal control group. The SW480 cells were treated with Tianma granule medicated serum (17.0 g/mL) in normal dose group, and Tianma granules medicated serum (8.5 g/mL) in 50% normal dose group. The SW480 cells were cultured with blank serum in blank serum group. After 24 hours, the expression of miR-34a was detected by RT-PCR, and the proliferation of cells was detected by MTT. The apoptosis of cells was detected by flow cytometry, and the expressions of p53 and cleaved Caspase-3 were detected by Western blotting. SW480 cells were divided into Tianma granules group, Tianma granules +NC inhibitor group, and Tianma granules+miR-34a inhibitor group. The SW480 cells were treated with drug-containing serum (17.0 g/mL) in Tianma granule group. The Tianma granule+NC inhibitor group and Tianma granule+miR-34a inhibitor group were transfected with NC inhibitor and miR-34a inhibitor, respectively, followed by intervention with drug-containing serum (17.0 g/mL) of Tianma granules. After 24 h, the expressions of p53 and cleaved Caspase-3 were detected by Western blotting. Results: The serum concentration of Tianma granules in vitro was 17.0 μg/mL. Tianma granule could inhibit the proliferation of SW480 cells, promote the apoptosis of SW480 cells, and up-regulate the expression of miR-34a, p53 and cleaved Caspase-3 in colorectal cancer cells. The regulatory effect on colorectal cancer cells was dose-dependent. Inhibition of miR-34a could reverse the pro-apoptosis and anti-proliferation effects of Tianma granules on colorectal cancer cells. Conclusion: Tianma granules can inhibit colorectal cancer cell proliferation and promote colorectal cancer cell apoptosis by up-regulating the expressions of p53 and cleaved Caspase-3 by up-regulating miR-34a.

Key words：colorectal cancer; Tianma granules; miR-34a; cell proliferation; cell apoptosis

发布时间：2025-12-20

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