黄芪多糖促进T细胞介导的抗肿瘤免疫功能研究*

作者：张海桐，郭子硕，杨凯丽，王晓静，陈宛灵，李加玲，阮明月，王一帆，杜守颖，李鹏跃

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引用：引用：张海桐，郭子硕，杨凯丽，王晓静，陈宛灵，李加玲，阮明月，王一帆，杜守颖，李鹏跃.黄芪多糖促进T细胞介导的抗肿瘤免疫功能研究[J].中医药导报,2025,31(4):8-13,20.

DOI：10.13862/j.cn43-1446/r.2025.04.002

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摘要：

目的：探究黄芪多糖（APS）对T细胞介导的抗肿瘤免疫增强作用。方法：通过慢病毒试剂转染，用绿色荧光蛋白（GFP）转染Lewis肺癌细胞（LLC），使LLC细胞带上绿色荧光，构建LLC-GFP荧光细胞株。取C57BL/6荷瘤小鼠的脾脏，在体外培养状态稳定的原代T细胞。采用活细胞分析系统绿色荧光模式，以LLC-GFP细胞的平均荧光强度为指标，考察APS对T细胞杀伤能力的增强作用。分别采用IncuCyteR活细胞分析系统和流式细胞术分析APS对原代T细胞增殖和表型转化的影响。结果：细胞杀伤实验结果显示，与空白组比较，对照组及给药组荧光强度随时间延长而逐渐降低，APS组（40 μg/mL）平均荧光强度最低，表明APS可提高T细胞对肺癌细胞的杀伤能力；细胞增殖实验表明，APS质量浓度为10、20、40 μg/mL时可促进原代T细胞的增殖；细胞表型实验显示，40 μg/mL的APS组原代T细胞CD8+细胞的比例为40.5%，高于对照组（37.6%），说明APS具有免疫调节的作用。结论：APS可以通过增加T细胞杀伤肺癌细胞的功能，增强机体的抗肿瘤免疫能力，实现抗肿瘤的作用。

关键词：肺癌；黄芪多糖；T细胞；免疫增强；抗肿瘤

Abstract：

Objective: To investigate the anti-tumor immune-enhancing effect of Astragalus polysaccharide (APS) on T cell-mediated immunity. Methods: The LLC-GFP fluorescent cell line was constructed by transfecting Lewis lung cancer cells (LLC) with green fluorescent protein (GFP) through lentiviral reagent transfection to bring green fluorescence to LLC cells. The spleen of C57BL/6 hormonal mice was taken, and the primary T cells in stable state were cultured in vitro. The green fluorescence mode of the live cell analysis system was used to investigate the enhancement of T cell killing ability by Astragalus polysaccharide, with the average fluorescence intensity of LLC-GFP cells as an index. The effects of Astragali polysaccharides on the proliferation and phenotypic transformation of primary T cells were analyzed by IncuCyteR Live Cell Analysis System and flow cytometry, respectively. Results: The results of cell killing experiments showed that compared with the blank group, the fluorescence intensity of the control group and the administered group decreased gradually with the increase of time, and that APS group (40 μg/mL) showed the lowest average fluorescence intensity, which indicated that APS could enhance the killing ability of T cells against lung cancer cells. The proliferation assay showed that APS could promote the proliferation of primary T cells with the concentration at 10, 20 and 40 μg/mL. The cell phenotype assay showed that the proportion of CD8+ cells in primary T cells was 40.5% in the APS group (40 μg/mL), which was higher than that in the control group (37.6%). It indicated that APS played a role in immunomodulation. Conclusion: Astragalus polysaccharide can enhance the anti-tumor immunity of the body and achieve anti-tumor effects by increasing the ability of T cells to kill lung cancer cells.

Key words：lung cancer; Astragalus polysaccharide; T cells; immune enhancement; anti-tumor

发布时间：2025-12-20

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