中药挥发油对消炎生肌散经皮促透作用研究*
作者：李 婷1，张国强2，王一男3
单位：1.徐州市中医院，江苏 徐州 221000； 2.徐州市中心医院，江苏 徐州 221000； 3.徐州医科大学药学院，江苏 徐州 221000
引用：引用：李婷，张国强，王一男.中药挥发油对消炎生肌散经皮促透作用研究[J].中医药导报,2025,31(11):51-55.
DOI：10.13862/j.cn43-1446/r.2025.11.009
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摘要：目的：探讨消炎生肌散中辅料冰片和薄荷挥发油对药效成分龙胆苦苷和盐酸小檗碱的体外最佳促透条件。方法：采用水蒸气蒸馏法提取消炎生肌散挥发性成分和薄荷挥发油；采用气相色谱内标法测定消炎生肌散挥发性成分中冰片的含量；通过Franz扩散池法进行体外透皮实验，建立高效液相色谱法测定接收液中龙胆苦苷和盐酸小檗碱含量；比较不同浓度的冰片和薄荷混合挥发油对龙胆苦苷和盐酸小檗碱的透皮吸收效果。结果：消炎生肌散挥发性提取物中冰片为主要成分，平均含量为30.36%；冰片和薄荷挥发油联用能促进龙胆苦苷、盐酸小檗碱的体外透皮吸收，1%薄荷挥发油+1%冰片对两者的促透效果最佳。结论：消炎生肌散中冰片、薄荷挥发油有协同促渗作用，具有“药辅合一”的特点。
关键词：消炎生肌散；挥发油；龙胆苦苷；盐酸小檗碱；透皮吸收
Abstract：
Objective: To investigate the optimal in vitro penetration conditions for the active ingredients (gentiopicroside and berberine hydrochloride) facilitated by the excipients (borneol and peppermint volatile oil) in the Xiaoyan Shengji Powder. Methods: Volatile components in Xiaoyanshengji Powder and peppermint volatile oil were extracted by steam distillation. The content of borneol in Xiaoyan Shengji Powder was determined by gas chromatography (GC) with the internal standard method. The transdermal absorption experiment was performed in vitro by Franz diffusion cells. High performance liquid chromatography (HPLC) was established to measure the content of gentiopicrin and berberine hydrochloride in the receiving solution, plotting drug-time curves, comparing the effects of different concentrations of mixed volatile oils of borneol and peppermint volatile oil on the transdermal absorption of gentiopicrin and berberine hydrochloride. Results: Borneol was the main component in volatile extracts of Xiaoyanshengji Powder with an average content of 30.36%. The combination of borneol and peppermint volatile oil promoted the in vitro transdermal absorption of gentiopicrin and berberine hydrochloride, and 1% borneol+1% peppermint volatile oil showed optimal penetration enhancement. Conclusion: Borneol and peppermint volatile oil in Xiaoyan Shengji Powder demonstrate synergistic effects on facilitating penetration.
Key words：Xiaoyan Shengji Powder; volatile oil; gentiopicrin; berberine hydrochloride; transdermal absorption
发布时间：2025-11-29
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