木犀草素通过cGAS/STING/NF-κB p65信号途径对慢性鼻窦炎大鼠炎症反应的影响*

作者：朱娅娴1，王 斐1，邓 魏1，杨晓倩1，朱镇华2

单位：1.湖南中医药大学，湖南 长沙 410208； 2.湖南中医药大学第一附属医院，湖南 长沙 410007

引用：引用：朱娅娴，王斐，邓魏，杨晓倩，朱镇华.木犀草素通过cGAS/STING/NF-κB p65信号途径对慢性鼻窦炎大鼠炎症反应的影响[J].中医药导报,2025,31(11):26-30,37.

DOI：10.13862/j.cn43-1446/r.2025.11.005

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摘要：目的：探讨木犀草素（Luteolin）调节环鸟甘酸腺苷酸合成酶（cGAS）/干扰素基因刺激因子（STING）/核因子κB p65(NF-κB p65）信号途径对慢性鼻窦炎（CRS）大鼠炎症反应的影响。方法：将大鼠随机分为Control组、Model组、Luteolin组、CLM组，每组10只。造模后观察大鼠挠鼻、打喷嚏、大鼠鼻窦黏膜形态等情况，随后取材；酶联免疫吸附试验（ELISA）检测血清白介素-1β（IL-1β）、白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、γ干扰素（IFN-γ）水平，HE染色检测各组大鼠鼻窦黏膜组织病理情况，蛋白质印迹法（Western blotting）、免疫荧光染色检测大鼠鼻窦黏膜组织中cGAS、STING、p65蛋白表达情况。结果：行为学及CT结果示，造模后大鼠挠鼻、打喷嚏症状明显（P＜0.01），大鼠上颌窦黏膜增厚，提示CRS大鼠模型构建成功；HE染色结果示，Model组大鼠上颌窦鼻黏膜组织上皮紊乱、细胞脱落、固有层嗜酸性粒细胞浸润、腺体肿胀和黏膜下血管充血，血清炎症因子IL-1β、IL-6、TNF-α及IFN-γ水平增加，cGAS、STING、p65蛋白表达增加（P<0.05）；与Model组比较，Luteolin组和CLM组大鼠鼻黏膜组织固有层嗜酸性粒细胞浸润减少、上皮排列有序，鼻黏膜细胞剥落减少，IL-1β、IL-6、TNF-α和IFN-γ降低，cGAS、STING、p65蛋白表达降低（P<0.05）。结论：木犀草素可能通过调节cGAS/STING/NF-κB p65信号途径对慢性鼻窦炎大鼠炎症反应起到改善作用。

关键词：

Abstract：

Objective: To investigate the effect of Luteolin on the inflammatory response in rats with chronic rhinosinusitis (CRS) by regulating the cyclic GMP-AMP synthase (cGAS)/stimulator of interferon genes (STING)/nuclear factor kappa B p65 (NF-κB p65) signaling pathway. Methods: Rats were randomly divided into Control group, Model group, Luteolin group, and CLM group, with 10 rats in each group. After modeling, rat behaviors such as nose scratching and sneezing were observed, and the morphology of the sinus mucosa was examined before sample collection. Serum levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) were detected by enzyme-linked immunosorbent assay (ELISA). Histopathological changes in the sinus mucosa of rats in each group were assessed by HE staining. Protein expressions of cGAS, STING, and p65 in the sinus mucosa were detected by Western blotting and immunofluorescence staining. Results: Behavioral and CT results showed that after modeling, rats exhibited significant nose scratching and sneezing (P<0.01), and the maxillary sinus mucosa was thickened, indicating successful establishment of the CRS rat model. HE staining results showed that in the Model group, the maxillary sinus mucosa exhibited disordered epithelium, cell exfoliation, eosinophil infiltration in the lamina propria, glandular swelling, and submucosal vascular congestion. Serum levels of inflammatory factors IL-1β, IL-6, TNF-α, and IFN-γ were increased, and protein expressions of cGAS, STING, and p65 were upregulated (P<0.05). Compared with the Model group, the Luteolin group and the CLM group showed reduced eosinophil infiltration in the lamina propria of the nasal mucosa, more orderly epithelial arrangement, decreased nasal mucosal cell exfoliation, lower levels of IL-1β, IL-6, TNF-α, and IFN-γ, and decreased protein expressions of cGAS, STING, and p65 (P<0.05). Conclusion: Luteolin may ameliorate the inflammatory response in rats with chronic rhinosinusitis by regulating the cGAS/STING/NF-κB p65 signaling pathway.

Key words：chronic rhinosinusitis; luteolin; cyclic GMP-AMP synthase (cGAS); stimulator of interferon genes (STING); nuclear factor kappa B p65 protein; inflammatory response; rats

发布时间：2025-11-29

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